Abstract

Agricultural production of Cicer arietinum demands, the use of nitrogen fertilizer as NH₄NO₃, its excessive application causes the loss of soil productivity and global warming by releasing N₂O. An ecological to solve this problematic condition could be: to isolate endophytic bacterial genera that promote plant growth such as: Azotobacter vinelandii and Burkholderia vietnamiensis, from Leucaena leucocephala a wild plant able to face nutritional environmental, that allow reducing and optimizing the dose of NH₄NO₃ at 50%, non-compromising healthy plant growth of C. arietinum. Therefore, the objectives of this work were: i) to isolate of endophytic genus of A. vinelandii and B. vietnamiensis from L. leucocephala, ii) to analyze the effect of A. vinelandii and/or B. vietnamiensis on growth of C. arietinum at 50% NH₄NO₃, iii) to identify A. vinelandii and B. vietnamiensis based on the biochemical profile. The isolation of A. vinelandii was in Burk agar and B. vietnamiensis on PCAT. The results showed that A. vinelandii and B. vietnamiensis isolated from L. leucocephala were beneficial to the healthy growth of C. arietinum by increasing the germination percentage and reducing the emergence time while both the phenology and biomass of C. arietinum were positively affected by 50% NH₄NO₃; based on the conversion of organic compounds in the root into phytohormones by A. vinelandii and/or B. vietnamiensis better when inoculated as a mixture than individually with numerical values that were statistically different by ANOVA-Tukey (P < 0.05), compared to the same numerical variables of C. arietinum growth with 100% NH₄NO₃ uninoculated, used as a relative control. While it was found that A. vinelandii showed a biochemical profile of this genus and species with cyst production while B. vietnamiensis had the biochemical profile of this genus and species. The above supports the idea that L. leucocephala is an important source of plant growth-promoting endophytic bacteria for sustainable agriculture without damaging the soil or generating N₂O to mitigate due to conventional agriculture production of C. arietinum.

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