Zeta Potential and Turbidimetry Analyzes for the Evaluation of Chitosan/Phytic Acid Complex Formation

Noura Saïed, Mohammed Aider


The aim of this work was to study the possible complex formation between chitosan and phytic acid. Zeta potential and turbidity measurements were used as a basis to confirm the possible complex between these two molecules. The obtained results showed that chitosan at a concentration of 0.1% (w/v) were soluble in 0.1% acetic acid solution. This concentration of the acetic acid was the lowest that allows chitosan to dissolve. A positive surface charge of chitosan was recorded in the pH interval from 1 to 7. The highest zeta potential values were obtained at pH < 5 and decreased significantly at pH 6 and 7. Regarding phytic acid, it was soluble in deionized water and acetic acid whatever the concentration of the acetic acid and in the entire pH range 2-10. Phytic acid had negative surface charge in deionized water and in 0.1% acetic acid, but was slightly positively charged in 5% acetic acid solution. The solubility of chitosan was decreased by the presence of phytic acid. The formation of chitosan/phytic acid complex as monitored by measuring the zeta potential does not allow us to conclude that the formation of this complex is possible.


chitosan, phytic acid, zeta potential, turbidity, complex

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DOI: https://doi.org/10.5539/jfr.v3n2p71


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Journal of Food Research   ISSN 1927-0887 (Print)   ISSN 1927-0895 (Online)  E-mail: jfr@ccsenet.org

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