Supplementation With Highly Purified Docosahexaenoic Acid Ethyl Ester Was Associated With Enhancement of Hepatic Fatty Acid Oxidation and Greater Apparent Absorption of Docosahexaenoic Acid in Rats

Kenji Fukunaga, Ryota Hosomi, Ryosuke Inayoshi, Toshimasa Nishiyama, Munehiro Yoshida


Fish oil-derived fatty acid ethyl ester (EE) has been widely utilized as a supplement in Europe and United States. However, few studies have assessed the apparent absorption of docosahexaenoic acid (DHA) between non-purified DHA-EE (L-DHAEE) and highly purified DHA-EE (H-DHAEE). This study evaluated the effects of L-DHAEE and H-DHAEE on lipid metabolism and apparent absorption of DHA in rats. Groups of male Wistar rats were fed AIN93G diet containing 2.8% L-DHAEE or 1.0% H-DHAEE for 4 or 8 weeks. As parameters of lipid metabolism and apparent absorption of DHA-EE, serum and liver lipid contents, fecal EE excretion, lipid-metabolizing enzyme activities, and organ fatty acid composition were measured. The L-DHAEE diet increased fecal DHA-EE and total EE excretion compared with the H-DHAEE diet. In rats fed DHA-EE for 4 weeks, H-DHAEE was more efficacious than L-DHAEE at increasing liver DHA content, partly owing to the enhancement of apparent absorption. In addition, rats fed the H-DHAEE diet had higher fatty acid ?-oxidation activity in peroxisomes than rats fed the L-DHAEE diet for 8 weeks. These results suggest that H-DHAEE supplementation may have beneficial functions in the prevention of lifestyle-related diseases as compared with L-DHAEE supplementation.


docosahexaenoic acid, fatty acid ethyl ester, apparent absorption, lipid metabolism

Full Text:




  • There are currently no refbacks.

Copyright (c)

Journal of Food Research   ISSN 1927-0887 (Print)   ISSN 1927-0895 (Online)  E-mail:

Copyright © Canadian Center of Science and Education

To make sure that you can receive messages from us, please add the '' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.