The Synthesis and Characterizes of Nano-Metallic Particles Against Antibiotic Resistant Bacteria, Isolated from Rasoul-e-Akram Hospital’s Patients, Tehran, Iran


  •  Alireza Jafari    
  •  Ali Majidpour    
  •  Roya Safarkar    
  •  Seyyedeh Masumeh Mirnurollahi    
  •  Shahrdad Arastoo    

Abstract

The emergence of antimicrobial resistance of microorganisms to antibiotics, Also, an increase in nosocomial infections, particularly by Methicillin Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, the need to discover new antibacterial agents with a mechanism of action different from killing bacteria were more than ever before. The Ag nanoparticles (NPs), ZnO (NPs) and Ag/ZnO (NPs) were synthesized through the thermal decomposition of the precursor of oxalate. Gram-negative antibiotic resistant bacteria and Gram-positive antibiotic resistant bacteria were prepared from the Central laboratory of Rasoul-e-Akram hospital. All of isolates were confirmed by biochemical tests. For determine of antibiotic resistance patterns of isolated, disk diffusion method in accordance with the standard CLSI were used, again. Antibacterial effects of (NPs) against antibiotic resistance bacteria were conducted by MIC and MBC tests. The particles size was less of 50 nm, approximately. Curiously, the silver (NPs) was not exposed the antibacterial properties against all of isolated bacteria. Also, klebsiella pneumonia and MRSA had greatest sensitivity to the ZnO (NPs). Also, Gram-positive antibiotic resistant bacteria showed high sensitivity to Ag/ZnO (NPs), compared to other bacteria. Interestingly, The MBC for ZnO (NPs) against Pseudomonas aeruginosa >= 8192 was observed. The Ag (NPs) had not the ability to inhibit the nosocomial infection. klebsiella pneumonia and MRSA had greatest sensitivity to the ZnO (NPs). Also, Gram-positive antibiotic-resistant bacteria showed high sensitivity to Ag/ZnO (NPs), compared to other bacteria. The Ag/ZnO (NPs) was ability to kill antibiotics resistant bacteria. The antibacterial agents can open a new leaf in our life in the treatment of nosocomial infections.



This work is licensed under a Creative Commons Attribution 4.0 License.
  • ISSN(Print): 1925-430X
  • ISSN(Online): 1925-4318
  • Started: 2011
  • Frequency: annual

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