Effect of TiO2 Nanoparticles on Antioxidant Enzymes Activity and Biochemical Biomarkers in Pinto Bean (Phaseolus vulgaris L.)
- Ahmad Ebrahimi
- Mohammad Galavi
- Mahmood Ramroudi
- Payam Moaveni
AbstractTests were done on the effects of titanium dioxide spray on Pinto bean (Phaseolus vulgaris L. c.v ‘c.o.s.16’). The study was conducted as a factorial experiment in a randomized complete block design with four replications for two years (2014 - 2015). Treatments consisted of two factors; the first factor was stage of plant growth that spraying was applied (rapid vegetative growth, flowering and pod filling); and the second factor was that of different concentrations of titanium dioxide nanoparticles (TiO2) that consisted of spray with water (control), nano titanium dioxide at concentrations of 0.01%, 0.02%, 0.03% and 0.05%. Activity of guaiacol peroxidase (GPX), activity of superoxide dismutase (SOD), activity of catalase (CAT), activity of peroxidase (POD), malonyldialdehyde (MDA) Content and 8-deoxy-2-hydroxyguanosine (8-OHDG) content were assayed. Results showed that effect of nano TiO2 was significant on activity of superoxide dismutase (SOD), activity of catalase (CAT), activity of peroxidase (POD), malonyldialdehyde (MDA) Content and 8-deoxy-2-hydroxyguanosine (8-OHDG) content. Results of combined analysis of variance showed that the effect year significantly affected on SOD and 8-OH-2-DG (P ≤ 0.05). The effect of different amounts of titanium dioxide nanoparticles (TiO2) significantly affected (P ≤ 0.05) on MDA and 8-OH-2-DG. The effects of different amounts of titanium dioxide nanoparticles and year were significant on SOD, POD, MDA and the amount of 8-deoxy-2-hydroxyguanosine in P ≤ 0.05. None of the physiological traits were affected by spraying of nano titanium dioxide. The effects of TiO2 nanoparticles times of spraying and year were significant on SOD, CAT and 8-deoxy-2-hydroxyguanosine (P ≤ 0.05). Interaction effects of nano TiO2 concentrations × nano TiO2 spraying times did not have a significant impact on SOD, CAT, POD, GPX, MDA and 8-OH-2-DG. Although, all trait were affected by interaction effects of year × nano TiO2 concentrations × nano TiO2 spraying times with the exception of GPX (P ≤ 0.05).
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