Ultra Structures Assessment and Comparison of Allergenic Features of Mature and Immature Pollens of Quercus persica L.
- Roya Zand
There are extensive Persian Oak forests in the west and south west of Iran. Since the pollens are one of the most plants allergenic factor, and 80 up to 90 percent of plant’s allergen is pollen based, therefore in the present study the allergenic features of Persian Oak’s mature and immature pollens were studied, using cello logy and anatomical methods. The pollen samples were fixed by FAA. SEM analysis showed spherical shaped pollen along with tricolpate and warty shape exine. Pollen’s extract achieved using salt phosphate buffer. Electrophoresis profile of proteins showed total 16 bands in the range of 16 to 116 Kd. Mature pollen also had one distinct 52 Kd band. Allergenic test using guinea pig (350-400 Kg and 4-6 old) carried out. The blood tests, based on the numbers of eosinophils, neutrophils and immunoglobulins content of sample and treatments showed significant differences to the control. The mature pollen extract also was more allergenic than that of immature.
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