Ectopic expression of dWarts containing multiple mutations in the insert segment caused enhanced phenotype in transgenic Drosophila

Yan Yan, Bin Kang

Abstract


dWarts is a NDR kinase that plays a key role in the regulation of cell proliferation and apoptosis. Loss of function of dwarts caused cell over-proliferation and cancer. In this study, one of regulatory mechanisms of dwarts was reported. Similar to human Lat1, the insertion segment of dwarts in the catalytic domain was identified as negative regulatory domain, as multiple mutations in the insertion segment by substituting the conserved alkaline amino acids resulted in the enhanced small compound eye phenotypes when expressed in the eye discs of Drosophila. Moreover, our results demonstrated that regardless of identical genetic background, both wild type dwarts and mutant dwarts could confer incomplete penetrance phenotype when ectopically expressed in the eye discs of transgenic Drosophila, suggesting multiple regulatory mechanisms may be associated with dwarts.


Full Text: PDF

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

Journal of Molecular Biology Research   ISSN 1925-430X (Print)   ISSN 1925-4318 (Online)

 

Copyright © Canadian Center of Science and Education

 

To make sure that you can receive messages from us, please add the 'ccsenet.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.