Effect of a 50-Hz Magnetic Field on the Degradation of Plasmid in the Presence of Cu(II) Ions and Hydrogen Peroxide

Yuuichi Kawashima, Honoka Fujii, Maresuke Nakayama, Masahiko Hasegawa, Satoru Shimanuki, Hidetake Miyata

Abstract


Power-line frequency (50/60 Hz) magnetic fields enhance DNA strand breaks in the cell. In order to understand the molecular mechanism underlying this phenomenon, we analyzed the conversion of plasmid from the supercoiled to the linear form. This conversion is promoted by hydroxyl radical generated by the reaction between Cu2+ and H2O2. The plasmid pHSG298 was incubated with 1 mM Cu2+ and 1 mM H2O2 and exposed to a 1.2 mT, 50-Hz magnetic field for 30, 60, and 90 min. The conversion of supercoiled DNA to linear DNA was analyzed by agarose gel electrophoresis. We found that exposure to the magnetic field for 90 min significantly enhanced the degree of conversion.


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DOI: https://doi.org/10.5539/ijc.v9n4p40

 

International Journal of Chemistry   ISSN 1916-9698 (Print)   ISSN 1916-9701 (Online)

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