Isolation and Structure Elucidation of Bioactive Compounds Chemical as Inhibitors of the Enzyme ?-Glucosidase Raru Bark Ethanol Extract (Vatica pauciflora Blume)

Ida Duma Riris, Tonel Barus, Partomuan Simanjuntak, Basuki Wirjosentono

Abstract


Inhibitory compound of ?-glucosidase bioactivity was isolated from ethanol extract of the stem bark of raru
(Vatica pauciflora Blume) which, is a wild plant that grows in Tapanuli state in Indonesia.The ethanol extract of
the stem bark of raru was partitioned and was chromatographed on columns with stationare fase silica gel 60
mesh F254 (0.063 mm-0.200 mm). The ethanol extract of isolates showed the enzyme ?-glucosidase inhibitor IC50
of 93.46. The next step is the determination of the chemical structure of the Ultraviolet–visible spectroscopy (UV
Vis); FT-IR (Fourier Transform Infrared spectroscopy), NMR (Nuclear Magnetic Resonance) namely COSY,
Heteronuclear Multiple-Quantum Correlation (HMQC); and Heteronuclear Multiple-Bond Correlation (HMBC).
It found of compounds consist of two of methoxy, one of aromatic, and one of carbonil compounds. The isolat is
a 3,4,9,Trihidroxy-2-(hydroxymethyl)-8-10-dimethoxy-2-3-4atetrahydropyrano(3,2-c)isochromen-6(10bH)-one
acording to International Union of Pure and Applied Chemistry (IUPAC).

Full Text: PDF DOI: 10.5539/ijc.v6n2p15

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International Journal of Chemistry   ISSN 1916-9698 (Print)   ISSN 1916-9701 (Online)

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