Immunomodulatory Activity of Polysaccharide from the Roots of Actinidia kolomikta on Macrophages

Di Guan, Zhenya Zhang, Yingnan Yang, Guoqing Xing, Jiqiang Liu

Abstract


Actinidia kolomikta is a species of wild plant that grows wildly in the northern part of Indochina. However there are no reports about its immunomodulatory activity. In this study, the immunomodulatory activities of crude polysaccharide (CP) fraction from the root of Actinidia kolomikta on macrophage RAW 264.7 cells were investigated. Results showed that treatment with 2.5-25 µg/ml of CP stimulated macrophage proliferation, nitric oxide production and phagocytosis in a dose-dependent manner. Treatment with 5 µM Doxorubicin (DOX) resulted in macrophages survival rate decrease (12.6%). However co-incubation with 25 µg/ml of CP, the DOX-induced macrophage survival rate was 89.8%. At the same time the apoptosis of macrophage decreased from 37.8% (5 µM DOX) to 21.5% (co-incubation with 10 µg/ml of CP). It is suggested that CP possesses immunomodulatory activity on macrophages and protects macrophages from DOX damage.


Full Text: PDF DOI: 10.5539/ijb.v3n2p3

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

International Journal of Biology   ISSN 1916-9671(Print)   ISSN 1916-968X  (Online)

Copyright © Canadian Center of Science and Education

To make sure that you can receive messages from us, please add the 'ccsenet.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.