Characterization and Prokaryotic expression of Glucuronyltransferase-S Gene in Silkworm Bombyx mori

Yukun Chen, KePing Chen, Fang Bao, Qin Yao

Abstract


As genome of B. mori is available in GenBank, identification of novel genes of B. mori can be carried out. In this study,
we used the in silico cloning method to obtain the Glucuronyltransferase-S (GlcAT-S) gene of B. mori and analysed
with bioinformatics tools. The result was confirmed by RT-PCR,prokaryotic expression and western blot. The GlcAT-S
cDNA contains a 843bp ORF and has three exons. The deduced protein has 280 amino acid residues, with the predicted
molecular weight of 31842. 02 Da, isoelectric point of 9.16, and contains conserved GlcAT domains. The protein shows
high degrees of identity with that of some homologous protein from other species.

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International Journal of Biology   ISSN 1916-9671(Print)   ISSN 1916-968X  (Online)

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