Purification and Characterization of Cellulase from Termite Ametermes eveuncifer (Silverstri) Soldiers

B. S. Fagbohunka, R. E. Okonji, Ayinla Zainab Adenike

Abstract


Cellulase enzyme was purified and characterized from termite soldiers (Ametermes eveuncifer) using 70% ammonium sulphate precipitation, ion exchange chromatography and affinity chromatography. The enzyme isolated had a specific activity of 5.04 U/mg with a percentage yield of 11.7%. The enzyme showed maximum activity at 500C and pH 8. The enzyme was not inhibited by Ba2+ at a concentration of 1mM and Pb2+ at 10 mM concentration but was inhibited by other metal ions at 1 mM and 10 mM concentrations of their salts (NaCl, KCl, MnCl2, and NiCl2),


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DOI: http://dx.doi.org/10.5539/ijb.v9n1p1

Copyright (c) BAMIDELE SANYA FAGBOHUNKA, RAPHAEL EMUEBIE OKONJI, ZAINAB ADENIKE AYINLA

License URL: http://creativecommons.org/licenses/by/4.0

International Journal of Biology   ISSN 1916-9671(Print)   ISSN 1916-968X  (Online)   Email: ijb@ccsenet.org

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