Detection of Conjugative Plasmid Encoded Ampicillin and Tetracycline Resistant in Klebsiella pneumonia

Abstract

Klebsiella pneumonia were isolated and identified on the basis of morphology, growth, and biochemical characteristics. All the isolates offered different resistance patterns against antibiotics including Ampicillin, Cefotaxime, Erythromycin, tetracycline and Chloramphenicol. Transformation and conjugation techniques were used for plasmid transfer studies. The conjugation experiment showed that ? 51kbp conjugative plasmid conferring resistance to tetracycline and ampicillins were successfully transferred to the recipient cells E. coli MM294. The rest of the plasmid borne markers was non-conjugative/nontransferable. Conjugative plasmids carry a tremendous potential to disseminate resistance markers to distant recipient cells. The protocol is reliable enough to be used for large-scale visualization of native plasmids and we have used it to visualize and isolate DNA from hundreds of multidrug resistance plasmids.