Characterization of a Differentially Expressed Phenylalanine Ammonia-Lyase Gene From Banana Induced During Mycosphaerella fijiensis Infection

Javier C. Alvarez, Hector Alejandro Rodriguez, Esperanza Rodriguez-Arango, Zulma I. Monsalve, Juan G. Morales O., Rafael E. Arango I

Abstract


Black leaf streak, caused by the fungus Mycosphaerella fijiensis, is the most important disease affecting the commercial production of banana and plantain in the world. Phenylalanine-ammonia-lyase (PAL) is an enzyme found in a large group of plants, which catalyzes the first reaction in the metabolic pathway of phenylpropanoid compounds. Phenylphenalenons, a type of phenylpropanoid metabolites, have been found in banana and have been suggested to be involved in resistance against pathogens. Thus, PAL might be an important enzyme in the plant-pathogen interaction. In this work, PAL cDNAs were isolated, and sequenced completely or partially, from different banana cultivars such as ‘Calcutta 4’ (Musaacuminata ssp. burmannicoides), ‘Grain Nain’ (AAA, Cavendish subgroup), ‘Yangambi Km5’ (AAA, Ibota subgroup) and ‘Williams’ (AAA, Cavendish subgroup). The obtained sequences were compared, and their secondary and three-dimensional structures of their deduced protein sequences were modeled. Several structural differences between cultivars were found. Additionally, expression analysis showed marked differences in the expression of PAL depending on the cultivar. In particular, PAL from black leaf streak- resistant ‘Calcutta 4’ showed highest expression in the early hours of infection in contrast to the susceptible ‘Williams’ cultivar.


Full Text: PDF DOI: 10.5539/jps.v2n2p35

Refbacks

  • There are currently no refbacks.


Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

Journal of Plant Studies   ISSN 1927-0461 (Print)   ISSN 1927-047X (Online)

Copyright © Canadian Center of Science and Education

To make sure that you can receive messages from us, please add the 'ccsenet.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.