Isolation of Endophytic Bacteria and Amplification of 16SrDNA on Citrus in Zhaoqing Districts

Chongbi Li, S. N. Liang, F. Y. Gao, K. N. Chen, J. Wang, M. Zhang, T. Sun, X. K. Mu

Abstract


Zhaoqing is the main source of Citrus in China. However, the reason of citrus Huanglongbing (HLB) is unclear and may be from endophyte. Therefore, the study of citrus endophyte is necessary. The experiment would be carried out by endophyte being isolated from citrus plant and amplifying the specific 16SrDNA fragment from endogenous bacteria to know the relation of citrus HLB to 16S rDNA of endogenous bacteria. The results showed that total 55 strains of endophytic bacteria were identified. And one specific fragment long of 1261 nt was got. Sequencing analysis showed for 91.3% homology to uncultured bacterium clone. And the 16SrDNA fragment was identified by PCR. The experiments would provide a theoretical basis for understanding the differences in pathomechanism among various HLB isolates.


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DOI: https://doi.org/10.5539/jas.v7n12p198

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Journal of Agricultural Science   ISSN 1916-9752 (Print)   ISSN 1916-9760 (Online)  E-mail: jas@ccsenet.org

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