Optimization of Ultrasound-Assisted Extraction of Flavonoids from Cryptotaenia japonica Hassk. and Evaluation of Antioxidant Activity

Jun Lu, Youzhi Xu, Mengxin Yang, Xinjing Fu, Feijun Luo, Zhonghai Li

Abstract


The present study optimized the ultrasound assisted extraction (UAE) conditions using Box-Behnken design (BBD) with response surface methodology (RSM) and evaluated the antioxidant activity of total flavonoids from Cryptotaenia japonica Hassk. The results showed that the optimum conditions were 55% ethanol concentration, 1:30 g/mL solid to solvent ratio, 400 W ultrasound power, 70 min extraction time and 3 cycles. Under these optimum conditions, the extraction yield of total flavonoids was 33.110 ± 0.742 mg/g DW (dry weight), which was well consisted with value predicted by the model. The RSM optimized extract was subsequently checked for antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, hydroxyl scavenging assay and reducing power assay. Under the flavonoid concentration of 3.0 mg/mL, antioxidant scavenging activity were of 79.87±2.7% and 71.60±2.6% for DPPH and hydroxyl assay while for reducing  power and absorbance of 1.200±0.053 was obtained. Furthermore, the composition in antioxidants were investigated, and the luteolin and apigenin were determined. The results indicated that ultrasound assisted extraction is a promising technique for extraction of flavonoids from C. japonica Hassk and the flavonoids could be explored as a potential antioxidant agent for use in medicine or functional foods.


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DOI: https://doi.org/10.5539/jas.v7n7p138

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