Optimization of Saccharification Conditions of Acid-pretreated Sweet Sorghum Straw Using Response Surface Methodology

Sukanya Phuengjayaem, Aphisit Poonsrisawat, Amorn Petsom, Siriluk Teeradakorn

Abstract


This study focused on the cellulase production from C. versicolor TD17, white rot fungi. The maximum cellulase activity of 0.897 U/ml was obtained after 5 days of cultivation using 20 g/l cellobiose as a carbon source and 2 g/l ammonium sulfate supplemented with 0.3 g/l urea as nitrogen sources. Enzymatic saccharification of acid-pretreated sweet sorghum straw (SSS) using in house cellulase was optimized using Response Surface Methodology (RSM), variable five-code-level, four-factor; 1.0-7.0% w/v acid-pretreated SSS, 15-35 FPU/g dry substrate of cellulase enzyme, pH 3 to 7 and temperatures 30 to 70 °C. The optimal conditions were 1% w/v acid-pretreated SSS, 25 FPU/g dry substrate of cellulase, pH 5, 50 °C and 72 h cultivation. A maximal glucose yield of 0.440 g/g dry substrate was obtained.


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DOI: http://dx.doi.org/10.5539/jas.v6n9p120

Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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