Comparison and Improvement of Different Methods of RNA Isolation from Strawberry (Fragria * ananassa)

Dingqun Yu, Haoru Tang, Yong Zhang, Zhen Du, Haowei Yu, Qing Chen

Abstract


Isolation of high-quality RNA from different strawberry tissues is often affected by the presence of high levels of contamination by polysaccharides and phenolic compounds. By the methods of improved CTAB, SDS, and guanidinium thiocyanate, total RNA was isolated from leaves of strawberry. The result indicated that total RNA is of high quality and undegraded by using CTAB. However, it was difficult to isolate total RNA from leaves of strawberry by using guanidinium thiocyanate, and total RNA could be extracted by using SDS with low concentration, impurity and degradation in some degree. After comparing, an improved 3% CTAB3 was used to isolate RNA from tissues of strawberry, and the isolated RNA was good enough for further applications. After RT-PCR, the 699 bp sequence of glucose-6-phosphate dehydrogenase (G6PDH) gene was obtained to identify the quality of total RNA from strawberry. In this experiment, improved 3% CTAB3 method could be regarded as a simple, rapid, economic and convenient method for the RNA isolated from strawberry.


Full Text: PDF DOI: 10.5539/jas.v4n7p51

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Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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