Abstract

A reliable protocol for callus induction and regeneration were developed for leaf explants of Artemisia vulgaris L. Percent of callus induction and regeneration were higher in the young leaves. MS medium containing 1.0 mgl^-1 BAP and 3.0 mgl^-1 NAA is the optimum concentration for induction of callus. So produced callus was subcultured on Murashige – Skoog (MS) medium with 1.0 mgl^-1 6-benzylaminopurine (BAP), 3.0 mgl^-1 gibberellic acid (GA₃) produced the highest mean number of shoots (35.85±0.81) per explant. Half strength of MS was found to be the best for rooting, however, addition of IAA (0.5-1.0 mgl^-1) was found essential to induce longer roots. More than 84% of the rooted plants were established in polycups after hardening.

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