Polymorphism of Lactoferrin Gene with PCR - RFLP and its Association with Subclinical Mastitis in Dairy Cows

Changhong Zhao, Gaoming He, Yanliang Wang, Zhaoxia Zhang


Incidence of mastitis was checked by the California Mastitis Test (CMT). 120 cows were screened and classified into 2 groups, 60 each group, namely control group (healthy) and experimental group ( subclinical mastitis). Polymorphism of cow lactoferrin gene promoter was determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The results showed that lactorferrin gene promoter was polymorphic among different groups, which indicated that its polymorphism was related to mastitis infection.

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DOI: https://doi.org/10.5539/mas.v3n2p144

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