Inhibitory Activity of Citrus Madurensis Ripe Fruits Extract on Antigen-induced Degranulation in RBL-2H3 Cells

Kimihisa Itoh, Kazuya Murata, Megumi Futamura-Masuda, Takahiro Deguchi, Yuko Ono, Marin Eshita, Masahiko Fumuro, Morio Iijima, Hideaki Matsuda

Abstract


The purpose of this study was to search edible ripe Citrus fruits which are applicable for functional food materials as juice, tea and/or jam with sweet taste and rich aroma. A fifty percent ethanolic extract (CMR-ext) obtained from the edible ripe fruit of Citrus madurensis exhibited an inhibitory activity of antigen-induced degranulation in anti-dinitrophenyl (DNP) IgE antibody sensitized rat basophilic leukemia (RBL) -2H3 cells. The inhibitory effect of the CMR-ext on degranulation in RBL-2H3 cells was attributable to 3’,5’-di-C-β-glucopyranosylphloretin (1) which is a constituent of C. madurensis. The effect of 1 on Akt and mitogen-activated protein kinases (MAPK) phosphorylation was examined in RBL-2H3 cells. Western blot analysis revealed that 1 (50 μM) inhibited the degranulation by suppression of Akt and p38 phosphorylation.


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DOI: https://doi.org/10.5539/jps.v6n1p23

Copyright (c) 2016 Kimihisa Itoh, Kazuya Murata, Megumi Futamura-Masuda, Takahiro Deguchi, Yuko Ono, Marin Eshita, Masahiko Fumuro, Morio Iijima, Hideaki Matsuda

License URL: http://creativecommons.org/licenses/by/4.0

Journal of Plant Studies   ISSN 1927-0461 (Print)   ISSN 1927-047X (Online)  E-mail: jps@ccsenet.org

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