A Simple Gold Nanoprobe Assay for the Identification of Staphylococcus Aureus, Listeria Monocytogenes and Salmonella Enteritidis in Food Specimens

Dimitra Panagiotis Houhoula, Ekatherina Charvalos, Spyros Konteles, Stamatis Koussissis, Vladimiros Lougovois, Joseph Papaparaskevas

Abstract


In the present study, we developed a gold nanoprobe assay, which relies on the colorimetric differentiation of specific DNA sequences, based on different aggregation profiles. We evaluated the assay on DNA extracted from pathogen cultures and from contaminated food specimens. The targets used were the femA gene for the identification of Staphylococcus aureus, the hly gene of the Listeria monocytogenes listeriolysin and the invA sequence for Salmonella spp. Comparison was performed with the reference assay, as described in the respective ISO guidelines for each pathogen, and a direct PCR amplification and detection. The minimum detection limit of the assay was defined at 123 fg/μL DNA, for all species, lower than PCR detection. Specificity was 100%. Sensitivity was 100%, as compared το the reference method, whereas the sensitivity of PCR was 93.3%. The evaluated assay could be used as a sensitive screening method, which does not require major infrastructure, for the detection and identification of pathogens in food specimens. In addition, it can accommodate detection of multiplespecies,thus allowing multiplexingand expedite turnaround time. 


Keywords


Gold nanoparticles, foodborne pathogens

Full Text:

PDF


DOI: https://doi.org/10.5539/jfr.v6n4p134

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 DIMITRA PANAGIOTIS HOUHOULA, Ekatherina Charvalos, Spyros Konteles, Stamatis Koussissis, Vladimiros Lougovois, Joseph Papaparaskevas

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Journal of Food Research   ISSN 1927-0887 (Print)   ISSN 1927-0895 (Online)  E-mail: jfr@ccsenet.org

Copyright © Canadian Center of Science and Education

To make sure that you can receive messages from us, please add the 'ccsenet.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.