Synthesis of DEAE-Soybean Starch Microspheres for Adhere Animal Cell Culture

Lei Zhang, Mingsheng Li, Zhongren Ma, Yuping Feng

Abstract


The present study outlines the synthesis of a new microcarrier for anchorage-dependent animal cell cultures. The new microcarriers were synthesized from the cross-linking soybean starch microspheres followed by modification with 2-diethylaminoethyl (DEAE). Furthermore, 5 g/100 mL of wet microspheres DEAE-soybean starch microspheres were applied in the adhere cell culture, with an inoculation density 2.0 × 105 cells/mL of BHK-21, Marc-145, and MDCK cells. The cells were shown to grow well in the DEAE-soybean starch microcarrier, with BHK-21 cells showing a higher cell density after 144 h (2.5 × 106 cells/mL) compared to cells grown on the commercial product Cytodex 1 (2.2 × 106 cells/mL). These starch microcarriers have a potential application in anchorage-dependent animal cells culture, due to its low cost and its simple process.


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DOI: https://doi.org/10.5539/jas.v9n8p91

Copyright (c) 2017 Lei Zhang

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Journal of Agricultural Science   ISSN 1916-9752 (Print)   ISSN 1916-9760 (Online)  E-mail: jas@ccsenet.org

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