Study Regarding the in Vitro Regeneration and Use Perspectives in Some Valeriana officinalis L. Genotypes

Dorin D. Camen, Carmen G. Beinsan, Radu L. Sumalan

Abstract


The purpose of the researchers was to evaluate the regeneration possibilities of the species Valeriana officinalis L. from the point of view of “in vitro” cultivation of the species, as well as the influence of genotypes and of the culture environment on the “in vitro” reaction of the species. The biological material used in the process of research was made up of cultivated and spontaneous forms of Valeriana officinalis L. present in the collection of the University of Agricultural Science and Veterinary Medicine in Cluj-Napoca, Romania. The studied genotypes were: Valeriana officinalis, Volgensis, Sambucifolia, Collina, Rossica, Magurele 100, Moscova 835 with different geographical provenience. For the “in vitro” culture of Valeriana officinalis L. explants stemming from 2-4 week-old plants were used. The explants were formed of fragments of stem measuring 0.2-0.5 cm and leaf fragments 0.4-0.6 cm in length and 0.3-0.5 cm wide. The results obtained were different, according to the hormone balance: callus or direct risogenesis from the inoculated explants. Also we tested the regenerative possibilities from cell suspension from callus and roots with future possibilities for volatile oils extraction from in vitro suspension. The culture environment used was Murashige-Skoog, 1962, with different hormonal variations as follows: V0 – Mt. Murashige-Skoog culture media; V1 – 10 mg/L naftalen acetic acid (NAA) and 0.1 mg/L benzyl amino purine (BAP); V2 – 1 mg/L NAA and 1mg/L BAP; V3 – 0.1 mg/L NAA and 1mg/L BAP.


Full Text: PDF DOI: 10.5539/jas.v4n8p222

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Journal of Agricultural Science ISSN 1916-9752 (Print) ISSN 1916-9760 (Online)

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