Immobilization of Aspergillus Oryzae B-galactosidase on Porous Magnetic Poly (GMA-DVB) Particles via Suspension Polymerization

Sufang Sun, Lulu Peng, Xuejie Ming

Abstract


The porous ferric GMA-DVB polymer was synthesized from glycidlymethacrylate (GMA) and divinyl benzene (DVB) by suspension polymerization with lauryl alcohol and cyclohexanol as pore-forming reagents in the presence of FeCl3 and FeCl2. The structure and the magnetism of the GMA-DVB polymer beads were featured with magnetic scales, X-ray spectroscopy and scanning electron microscope (SEM). Aspergillus Oryzae B-galactosidase was bounded on the magnetic polymer under the best terms, and the maximum activity was determined to be 271.25U/g dry carries. Meanwhile, the fundamental properties about the bounded enzyme, such as the influence of temperature and pH, were assayed and the satisfactory results were got.


Full Text: PDF DOI: 10.5539/ijc.v5n4p38

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International Journal of Chemistry   ISSN 1916-9698 (Print)   1916-9701 (Online)

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