Optimization of Subcritical Water Extraction of Polysaccharides from Inonotus Obliquus and their Antioxidant Activities


  •  Xi Yuan    
  •  Ling Li    
  •  Hongyi Sun    
  •  Shuang Sun    
  •  Zhenya Zhang    

Abstract

Subcritical water extraction (SWE) of Inonotus Obliquus polysaccharides (IOP) was investigated using response surface methodology (RSM) with a design by Box–Behnken design (BBD). Results showed that the optimum SWE conditions for IOP production were as follows: extraction temperature 194°C, residence time 5.36 min and liquid-solid ratio 53 mL/g, yielding 168.80 ± 0.59 mg/g of IOP, which was in close agreement with the values predicted by the mathematical model. FT-IR spectra of the polysaccharides extracted by SWE and hot water extraction (HWE) were compared as well. Moreover, in vitro antioxidant assays revealed that SWE-IOP exhibited stronger scavenging activity that HWE-IOP. This investigation suggests that polysaccharides of Inonotus Obliquus extracted by SWE could be further developed as a potential antioxidant resource for dietary supplements of functional food.



This work is licensed under a Creative Commons Attribution 4.0 License.
  • Issn(Print): 1916-9671
  • Issn(Onlne): 1916-968X
  • Started: 2009
  • Frequency: quarterly

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