A hyaluronidase from the Snake Venom of Agkistrodon Blomhoffii Ussurensis of Changbai Mountain: Isolation and Characterization


  •  Dubo Zhong    
  •  Qingxiong Meng    
  •  Junming Li    
  •  Yaqin Wang    
  •  Xiaohong Zhang    
  •  Shixing Wang    
  •  Hongkun Zhu    

Abstract

Snake venom is a complex mixture of protein and peptide toxins. Hyaluronidase a‘spreading factor’ has not been
studied extensively in snake venom. In this paper, we describe the purification and characterization of a
hyaluronidase from the snake venom of Agkistrodon blomhoffii ussurensis of Changbaimountain. Hyaluronidase
has been purified by the successive chromatography on a DEAE-Sephadex A-25, on CM- Sephadex C-25 and on
Sephadex G-75 columns on the basis of the purification method of thrombin-like enzyme (TLE). The molecular
mass was found to be 35.0 kDa by SDS-polyacrylamide gel electrophoresis. Hyaluronidase was 5.66 percent of
the snake venom of Agkistrodon blomhoffii ussurensis of Changbai mountain, the activity of mass (u/g) was
437218.5. The pH optimum for the enzyme was in the rang of 3.0-4.0 and 6.0-6.5,maximum activity was
obtained at 37?, and the optimum concentration of Na+(mol/L) was in the rang of 0.3-3.0, the Km for
hyaluronidase, using hyaluronic acid as substrate, was 4.633 × 10-3 mg/ml. The hyaluronidase had not the
inhibition effect to growth of the Hela cell and HepG-2 cell in vitro through the experiment.


This work is licensed under a Creative Commons Attribution 4.0 License.
  • Issn(Print): 1916-9671
  • Issn(Onlne): 1916-968X
  • Started: 2009
  • Frequency: quarterly

Journal Metrics

Google-based Impact Factor (2017): 13.63
h-index (January 2018): 22
i10-index (January 2018): 75
h5-index (January 2018): 13
h5-median (January 2018): 16

Learn More

Contact