Preservation and Transportation of Hepatocyte Monolayers in A No Spill Format at Non Freezing Temperatures


  •  Peter Evans    

Abstract

Hepatocyte monolayers were preserved under a gelatin overlay at 10°C.   Morphological changes were observed after 4 days, as structure less areas at the cell periphery.   The changes could be prevented by adding sucrose (0.05M) to the gel.   Cell viability remained high for 7 days.   Cell spreading occurred rapidly when the cells were reactivated.   Basal enzyme levels were maintained during the preservation period.    The hormonal induction of tyrosine aminotransferase was stable for 4 days but declined subsequently.   Sucrose addition extended the period of preservation where induction was evident.  The method provides a robust system that allows preservation in the absence of potentially deleterious chemicals.   Time is allowed to send cells anywhere in the World in a no spill format whist arriving at their destination as if freshly produced.



This work is licensed under a Creative Commons Attribution 4.0 License.
  • Issn(Print): 1916-9671
  • Issn(Onlne): 1916-968X
  • Started: 2009
  • Frequency: quarterly

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