Preservation and Transportation of Hepatocyte Monolayers in A No Spill Format at Non Freezing Temperatures

Peter J. Evans

Abstract


Hepatocyte monolayers were preserved under a gelatin overlay at 10°C.   Morphological changes were observed after 4 days, as structure less areas at the cell periphery.   The changes could be prevented by adding sucrose (0.05M) to the gel.   Cell viability remained high for 7 days.   Cell spreading occurred rapidly when the cells were reactivated.   Basal enzyme levels were maintained during the preservation period.    The hormonal induction of tyrosine aminotransferase was stable for 4 days but declined subsequently.   Sucrose addition extended the period of preservation where induction was evident.  The method provides a robust system that allows preservation in the absence of potentially deleterious chemicals.   Time is allowed to send cells anywhere in the World in a no spill format whist arriving at their destination as if freshly produced.


Full Text: PDF DOI: 10.5539/ijb.v6n2p58

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International Journal of Biology   ISSN 1916-9671(Print)   ISSN 1916-968X  (Online)

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