The Effects of N-(4-hydroxyphenyl) Retinamide on Proliferation and Apoptosis of Hela Cells

Xiao-Hong Han, Yan-Jun Xue, Shi-He Shao, Xian-Qian Li, Hua-Xi Xu

Abstract


To investigate the effects of N-(4-hydroxyphenyl) retinamide (4HPR) on the proliferation and apoptosis of Hela cells, the cell growth was observed by SRB test, colony-forming test and nude mice carcinogenic test. Morphologic changes of apoptosis were observed under microscopes and the normal, apoptotic and necrotic cells were identified by fluorescence staining. The biochemical features and percentage of apoptosis were performed by flow cytometry (FCM) and DNA agarose gel electrophoresis. The results of SRB test, colony-forming test and nude mice carcinogenic test showed that 4HPR could inhibit the cells proliferation in vitro and in vivo (P<0.01). The apoptotic changes and apoptosis bodies could be observed under microscopes. The fluorescence staining revealed that 4HPR could induce the cells apoptosis, not necrosis. Typical DNA ladder appeared in 4HPR-treated cells and detected by FCM, the subdiploid nuclear peak appeared on the left of G1 peak and the apoptotic percentage was correlated with 4HPR in a dose and time dependent manner(P<0.01). All these results indicated that 4HPR could inhibit the proliferation of Hela cells and induce the cells apoptosis.


Full Text: PDF DOI: 10.5539/ijb.v3n3p161

Creative Commons License
This work is licensed under a Creative Commons Attribution 3.0 License.

International Journal of Biology   ISSN 1916-9671(Print)   ISSN 1916-968X  (Online)

Copyright © Canadian Center of Science and Education

To make sure that you can receive messages from us, please add the 'ccsenet.org' domain to your e-mail 'safe list'. If you do not receive e-mail in your 'inbox', check your 'bulk mail' or 'junk mail' folders.