Investigate the Effect of Nd-Yag Laser Beam on Soybean (Glycin max) Leaves at the Protein Level


  •  Noha Khalifa    
  •  H. Ghandoor    

Abstract

The effect of pre-sowing laser treatment on soybean seeds was investigated, using neodymium-doped yttrium
aluminium garnet (Nd-Yag) laser source at wave length lamda= 532nm. Soybean uniform seeds (Glycin max L. cv.
Giza 111) were exposed to laser irradiation for 5, 10, 30, 60 and 120 minutes. The growth potential of 4 - weekold
seedlings was progressively enhanced with the increase in time durations of laser treatment. In this study,
protein banding patterns of the leaf pools of the irradiated and non-irradiated control plants were investigated to
elucidate the role of laser at the molecular level. These patterns showed proportional increased levels of a ~ 55
KD protein in alliance with the pre - sowing exposure time to laser irradiation. This protein was gel eluted and
identified using de novo protein sequencing analysis of Liquid Chromatography - Mass Spectrometry and
Tandem Mass Spectrometry (LC/ MS/MS) technique. The database search of the results led to identification of
this protein as the large subunit of the enzyme 1,5 ribulose bisphosphate carboxylase/ oxygenase (RubisCO). The
concomitantly enhanced growth potential of soybean seedlings with the elevation of the level of RubisCO will
be discussed from different points of views.



This work is licensed under a Creative Commons Attribution 4.0 License.
  • Issn(Print): 1916-9671
  • Issn(Onlne): 1916-968X
  • Started: 2009
  • Frequency: quarterly

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