Community Shift of Methane-oxidizing Bacteria in Cover Soil of Waste Landfills Due to Methane Emission

Tomonori Ishigaki, Hiromi Sawamura, Kaoru Ikeda, Masato Yamada

Abstract


Methane oxidation at landfill surfaces was characterized using both physiological and genetically -based assessments of bacterial community. Two different types of methane oxidation were observed, depended on one of which involved separate methane-oxidizing reaction that were dependent on the initial methane concentration, and the other one involved normal Michaelis-Menten type kinetics. The MOB community structure was assayed by culture-independent molecular microbiological methods. The gene amplified by pmoA primer, as a measure of the total MOB population, was detected in levels of 104 to 105 MPN-copies•g-1, and accounted for 2.9 % - 49 % of the eubacterial 16S rDNA. In soils exposed to high methane flux, the type II MOB was predominated showing the value of 104 MPN-copies•g-1 of the mmoX gene and amounted to 30-90 % of the corresponding pmoA gene copies detected. In contrast, type I MOB existed in relatively high population at soils under the negative/negligible methane flux.

Full Text: PDF DOI: 10.5539/ep.v1n1p75

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Environment and Pollution   ISSN 1927-0909 (Print)   ISSN 1927-0917 (Online)

 

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