Screening and Identification of a Quercetin-Producing Bacterium Paenibacillus glucanolyticu D3

Abstract

Quercetin is a kind of flavonoid, which has outstanding drug action. To develop the production technology of quercetin by microorganism, strains that can transform rutin to quercetin were isolated from soil, screened by TLC and re-screened by HPLC. As a result, strain D3 with the highest transformation ability was obtained. The hydrolyze enzyme was intracellular enzyme and transformation rate was 41.6% within a system comprising of 50mg wet cells in 4mL of 0.1% rutin solution and transformed for 72hr at 25°C. Strain D3 was identified as Paenibacillus glucanolyticus by integrating the 16S rDNA phylogeny analysis result and a variety of morphological, physiological and biochemical features.